FFES/91215 Overview: Estradiol Free, Serum (includes Estradiol and SHBG)

Test Catalog

Test Name

Normal View

Estradiol Free, Serum (includes Estradiol and SHBG)

Method Name A short description of the method used to perform the test

High-pressure liquid chromatography/tandem mass spectrometry (HPLC/MS-MS);Electrochemiluminescence immunoassay (ECLIA)

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

Free Estradiol with SHBG

Aliases Lists additional common names for a test, as an aid in searching

Estradiol, Free, Serum FORWARD
Free Estradiol, Serum FORWARD

Specimen Type Describes the specimen type validated for testing

Serum Red

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Draw blood in a plain red-top tube (serum gel tube is not acceptable). Spin down, pour off into plastic vial within 1 hour of collection, and send 3 mL serum frozen.

Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

1.5 mL Note: This volume does not allow for repeat testing.

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected











Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperatures are also included.

Specimen TypeTemperatureTime
Serum RedFrozen (preferred)180 days
 Ambient 48 hours
 Refrigerated 48 hours

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.

Estradiol, Serum MS

Units: pg/mL


       Age                       Range


Levels are markedly elevated at birth and fall rapidly during the first week to prepubertal values of <15.


Males <6 m

Levels increase to 10 - 32 between 30 and 60 days, then decline to prepubertal levels of <15 by six months.


Females <1 y

Levels increase to 5.0 - 50 between 30 and 60 days, then decline to prepubertal levels of <15 during the first year.


Prepubertal                   <15

Adult Males                   8.0 - 35

Adult Females

 Follicular                      30 - 100

 Luteal                          70 - 300

 Postmenopausal          <15


Free Estradiol, Percent

Units: %


      Age                        Range

Adult Males                 1.7 - 5.4

Adult Females             1.6 - 3.6


Free Estradiol, Serum

Units: pg/mL


      Age                        Range

Adult Males                 0.2 - 1.5

Adult Females             0.6 - 7.1


Sex Hormone Binding Globulin

Units: nmol/L


      Age                                    Range

 Infants (1 - 23m)                  60.0 - 252.0

Prepubertal                          72.0 - 220.0


  Males                               16.0 - 100.0

  Females                            36.0 - 125.0

Adult Males

   20 - 49 y                           16.5 - 55.9

   >49y                                 19.3 - 76.4

Adult Females

   20 – 49y                            24.6 - 122.0

   >49y                                 17.3 - 125.0


PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.


Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

3 - 5 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

5 - 9 days

Performing Laboratory Location The location of the laboratory that performs the test

Esoterix Endocrinology

CPT Code Information Provides guidance in determining the appropriate Current Procedural Terminology (CPT) code(s) information for each test or profile. The listed CPT codes reflect Mayo Medical Laboratories interpretation of CPT coding requirements. It is the responsibility of each laboratory to determine correct CPT codes to use for billing.

CPT codes are provided by the performing laboratory.

82670 - Estradiol

84270 - Sex Hormone binding globulin

84999 - Unlisted chemistry procedure

LOINC® Code Information Provides guidance in determining the Logical Observation Identifiers Names and Codes (LOINC) values for the order and result codes of this test.

LOINC codes are provided by the performing laboratory.

Test IDTest Order NameOrder LOINC Value
FFESFree Estradiol with SHBGIn Process


Result IDTest Result NameResult LOINC Value
Z0259Estradiol, Serum35384-7
Z0228Free Estradiol, Percent14960-9
Z0229Free Estradiol, Serum2240-0
Z0230Sex Hormone Binding Globulin (SHBG)13967-5