AFPA - Specimen: Alpha-Fetoprotein, Amniotic Fluid

Test Catalog

Test Name

Test ID: AFPA    
Alpha-Fetoprotein, Amniotic Fluid

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

If alpha-fetoprotein (AFP) is positive, then acetylcholinesterase (AChE) will be performed at an additional charge.

Specimen Type Describes the specimen type validated for testing

Amniotic Fld

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Container/Tube: Amniotic fluid container

Specimen Volume: 1 mL

Collection Instructions: Do not centrifuge.

Additional Information:   

1. The following information is required:

a. Date ultrasound performed

b. Estimated due date by ultrasound

c. Collection date

d. Gestational age must be between 13 and 24 weeks; 16 to 18 weeks preferred.

2. If chromosome studies are also requested, see AF / Chromosome Analysis, Amniotic Fluid. The specimen for AFP-AF testing, when requested with chromosome analysis, cannot be frozen.


Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected









Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperatures are also included.

Specimen TypeTemperatureTime
Amniotic FldRefrigerated7 days