VIRNR - Specimen: Viral Culture, Non-Respiratory

Test Catalog

Test Name

Test ID: VIRNR    
Viral Culture, Non-Respiratory

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

All routine viral cultures are inoculated into cell culture tubes for viral detection. The most common specimens received for routine testing include body fluid, rectal, spinal fluid, and stool. A rapid (16-hour incubation) shell vial cell culture assay will be inoculated when specimens are designated for herpes simplex virus (HSV) or cytomegalovirus (CMV) detection, or as appropriate for source indicated.

 

Blood, lymph node tissue, and bone marrow/bone tissue specimens are not good sources for viral culture and are frequently toxic to cell culture lines. Most molecular methods are appropriate for these specimen types (exception: bone tissue). See individual real-time PCR assay descriptions.

 

Testing information pertaining to specific sources:

Dermal specimens are only acceptable for enterovirus. Clearly indicate "Enterovirus" on test request.

 

Ocular fluids (vitreous and aqueous): viral culture is not recommended due to usually inadequate volumes. PCR testing is recommended. See individual real-time PCR assay descriptions.

 

Genital, synovial fluid, wound swab or tissue (includes pus, drainage, or abscess fluid) specimens are not acceptable for viral culture.

Specimen Type Describes the specimen type needed for testing

Varies

Advisory Information

Source-based recommendations for testing:

Dermal specimens: Leg, arm, skin, axilla, etc: 

Order LHSV / Herpes Simplex Virus (HSV), Molecular Detection, PCR and/or LVZV / Varicella-Zoster Virus, Molecular, Detection, PCR or LHSVZ / Herpes Simplex Virus (HSV) and Varicella-Zoster Virus (VZV), Molecular Detection, PCR

 

Genital specimens: Cervical, endocervical, genital, labia, penis, perianal, scrotum, vaginal

Order LHSV / Herpes Simplex Virus (HSV), Molecular Detection, PCR

 

Oral specimens: Gum, lip, mouth, palate, tongue, tonsil, parotid, buccal 

Order VRESP / Viral Culture, Respiratory

 

Respiratory specimens: Bronchoalveolar lavage, bronchial wash, lung, lung tissue, nasal swab, nasal wash, sputum, throat swab, tracheal aspirate 

Order VRESP / Viral Culture, Respiratory

 

Infectious agent-based recommendations for testing:

If cytomegalovirus (CMV) is suspected in bone marrow or urine specimens, order LCMV / Cytomegalovirus (CMV), Molecular Detection, PCR 

 

If herpes simplex virus (HSV) is suspected in a neonatal patient (<1 month), order VHSV / Herpes Simplex Virus (HSV), Culture From Neonates

 

State Health Department testing only: specimens will not be accepted at Mayo Medical Laboratories for the following diseases (submit directly to your state health department).

-Measles

-Mumps

-High-risk infectious agents (examples of high-risk infections agents include: Ebola and other causative agents of viral hemorrhagic fever, avian influenza, severe acute respiratory syndrome (SARS), and Middle Eastern respiratory syndrome coronavirus (MERS-CoV)

Shipping Instructions

Specimen must be transported at refrigerate temperature and swab specimens should be sent in viral transport media.

 

Specimens must be received and cultured in the laboratory within 7 days postcollection.

 

Specimens for viral culture should be transported to the laboratory as soon as possible for optimal recovery.

Necessary Information

Specimen source is required.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Specimen Type: Body fluid

Sources: Pericardial, peritoneal, amniotic

Container/Tube: Sterile container

Specimen Volume: 1 mL

 

Specimen Type: Rectal

Supplies:

Swab, Sterile Polyester (T507)

M5 Media (T484)

Container/Tube: Dacron-tipped swab with plastic handle (T507)

Specimen Volume: Swab

Collection Instructions: Place swab in M5 (T484) or other viral transport media.

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 1 mL

 

Specimen Type: Stool

Supplies: Stool Collection Kit, Random (T635)

Container/Tube: Sterile container

Specimen Volume: 5-10 g

 

Specimen Type: Tissue

Sources: Brain, colon, kidney, liver, etc.

Supplies: M5 Media (T484)

Container/Tube: Sterile container with 1 to 2 mL of sterile saline or multi-microbe medium (M5) (T484)

Specimen Volume: Entire collection

 

Specimen Type: Dermal (for enterovirus only)

Supplies:

Swab, Sterile Polyester (T507)

M5 (T484)

Container/Tube: Dacron-tipped swab with plastic handle (T507)

Specimen Volume: Swab

Collection Instructions:

1. Place swab in M5 (T484) or other viral transport media.

2. Clearly label "enterovirus" to ensure proper handling and test setup.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).

Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

Body Fluid or Spinal Fluid: 1 mL; Stool: 5 g; Urine: 0.5 mL; Tissue Biopsy: 5 mm

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Gel swab, swab with wood handle, E-swab

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
VariesRefrigerated7 days