BARRP - Specimen: Bartonella, Molecular Detection, PCR

Test Catalog

Test Name

Test ID: BARRP    
Bartonella, Molecular Detection, PCR

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

See Infectious Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology in Special Instructions.

Specimen Type Describes the specimen type validated for testing

Varies

Advisory Information

BART / Bartonella Antibody Panel, IgG and IgM, Serum and Warthin-Starry tissue stain (PATHC / Pathology Consultation) should be considered if PCR is negative and there is a strong suspicion of disease caused by these organisms.

Necessary Information

Specimen source is required.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Bartonella species DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Specimen Type: Fresh tissue or biopsy

Sources: Heart valve, liver, lymph node, or spleen

Container/Tube: Sterile container

Specimen Volume: Entire collection or 5 mm(3)

Collection Instructions: Collect fresh tissue specimen.

Specimen Stability Information: Refrigerated (preferred) <7 days/ Frozen <7 days  

 

Supplies: Tissue Block Container (T553)

Specimen Type: Paraffin-embedded tissue block

Sources: Heart valve, liver, lymph node, or spleen

Container/Tube: Sterile container for cut sections or Tissue Block Container (T553)

Specimen Volume: Submit formalin-fixed, paraffin-embedded tissue block to be cut and returned. Alternatively, perform cuts and place two to five 10-micron sections in a sterile container for submission

Specimen Stability Information: Ambient (preferred)/Refrigerated

 

Specimen Type: Fluid

Sources: Cerebrospinal or ocular (eg, vitreous humor fluid)

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Specimen Stability Information: Refrigerated (preferred) <7 days/Frozen <7 days

 

Specimen Type: Synovial fluid

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Pink top (EDTA), royal blue top (EDTA), sterile vial containing EDTA-derived aliquot, red clot tube (no anticoagulant), or sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Send specimen in original tube (preferred).

Specimen Stability Information: Refrigerated <7 days (preferred)/Frozen <7 days

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)

Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

Fresh tissue or biopsy: 5 mm(3)
Paraffin-embedded tissue block: two 10-micron sections
Fluid: 0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Tissue in formalin, formaldehyde, or acetone; anticoagulants other than EDTA, green top (heparin) tube, blue top (citrate) tube, yellow top (ACD) tube, serum separator tube (SST); slides; bone marrow

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperatures are also included.

Specimen TypeTemperatureTime
VariesVaries