ACASM - Specimen: Pernicious Anemia Cascade

Test Catalog

Test Name

Test ID: ACASM    
Pernicious Anemia Cascade

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

If vitamin B12 is <150 ng/L, then intrinsic factor blocking antibody (IFBA) is performed. If IFBA is negative or indeterminate, then gastrin is performed.

If vitamin B12 is 150 to 400 ng/L, then methylmalonic acid (MMA) is performed. If MMA is >0.40 nmol/mL, then IFBA is performed.

 

See Vitamin B12 Deficiency Evaluation in Special Instructions.

Specimen Type Describes the specimen type validated for testing

Serum

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Specimen Volume: 4 mL

Collection Instructions:

1. Fasting (8 hours)

2. Divide specimen into 3 plastic vials, 1 containing 1 mL, 1 containing 1.5 mL, and 1 containing 1.5 mL

3. Band specimens together.

Additional Information: This test should not be ordered on patients who have received vitamin B12 injection within the last 2 weeks.

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)

Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

2.3 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

NA

Other

NA

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperatures are also included.

Specimen TypeTemperatureTime
SerumFrozen14 days