HPFH - Specimen: Hemoglobin F, Red Cell Distribution, Blood

Test Catalog

Test Name

Test ID: HPFH    
Hemoglobin F, Red Cell Distribution, Blood

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

The percent of the patient's hemoglobin F is very important to the clinical utility of this test. HBF / Hemoglobin F, Blood will be performed at an additional charge to determine if Hemoglobin F, Red Cell Distribution testing is warranted. If the hemoglobin F value is normal for age or if the value is less than 5%, the red cell distribution test will not be performed. Samples with hemoglobin F (Hb F) levels above 35% will be accepted but are discouraged due to decreased test specificity. For these markedly elevated Hb F levels, a hemoglobin electrophoresis profile with request for beta globin gene molecular testing for elevated Hb F levels is recommended.


For information on thalassemias and appropriate test ordering, see Thalassemia/Hemoglobinopathy Patient Information in Special Instructions.

Specimen Type Describes the specimen type validated for testing

Whole Blood EDTA

Advisory Information

This test is a flow cytometry assay that determines the distribution of hemoglobin F (Hb F) within red blood cells. The HGBCE / Hemoglobin Variant, A2 and F Quantitation, Blood test is intended for monitoring purposes, such as the increase in Hb F after therapy, or the levels of hemoglobin variants after transfusion.


This test should not be ordered for fetal-maternal bleed. If fetal maternal bleed assessment is desired, see FMB / Fetomaternal Bleed, Flow Cytometry, Blood.

Necessary Information

1. Indicate the percent of hemoglobin F detected by hemoglobin electrophoresis. If this is not indicated, we will perform HBF / Hemoglobin F, Blood.

2. Indicate the reason for testing.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Container/Tube: Lavender top (EDTA)
Specimen Volume:
3 mL


If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)

Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected


Mild reject; Gross reject


Mild OK; Gross OK





Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperatures are also included.

Specimen TypeTemperatureTime
Whole Blood EDTARefrigerated7 days