PNRP - Specimen: Pneumocystis jiroveci, Molecular Detection, PCR

Test Catalog

Test Name

Test ID: PNRP    
Pneumocystis jiroveci, Molecular Detection, PCR

Specimen Type Describes the specimen type validated for testing


Shipping Instructions

Specimen must arrive within 7 days of collection; specimen >7 days will be rejected.

Necessary Information

Specimen source is required.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Pneumocystis species DNA is unlikely.


Submit only 1 of the following specimens:



Specimen Type: Body fluid

Sources: CSF or pleural

Container/Tube: Sterile container

Specimen Volume: 1 mL


Specimen Type: Respiratory

Sources: Bronchoalveolar lavage, bronchial washing, or sputum

Container/Tube: Sterile container

Specimen Volume: 1 mL


Specimen Type: Tissue

Sources: Respiratory

Container/Tube: Sterile container

Specimen Volume: 5-10 mm

Collection Instructions:

1. Submit fresh tissue.

2. Keep tissue moist with sterile water or sterile saline



Specimen Type: NALC/NaOH-digested respiratory specimens

Sources: Lavage fluid, bronchial washing, respiratory fluid, sputum, or tracheal secretion

Container/Tube: Sterile container

Specimen Volume: 2 mL

Collection Instructions:

1. Submit digested specimen treated with NALC/NaOH.

2. Clearly indicate on container and order form that specimen is a digested specimen.


If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (

Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

0.5 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected








Body fluid other than CSF or pleural fluid, blood, bone, non-respiratory tissue, bone marrow, organ tissues other than lung, paraffin-embedded tissue, specimen in anaerobe vial or viral transport medium, stool, swab, tissue in formalin fluid, urine, specimen >7 days old

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperatures are also included.

Specimen TypeTemperatureTime
VariesRefrigerated (preferred)7 days
 Frozen 7 days