STL - Specimen: Enteric Pathogens Culture, Stool

Test Catalog

Test Name

Test ID: STL    
Enteric Pathogens Culture, Stool

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex tests may be performed and charged separately. Includes Aeromonas, Campylobacter, Salmonella, Shigella, and Yersinia.

Note: Does not include culture for Escherichia coli O157:H7.

 

See Laboratory Testing for Infectious Causes of Diarrhea in Special Instructions.

Specimen Type Describes the specimen type needed for testing

Fecal

Advisory Information

1. If Vibrio is suspected, order VIBC / Vibrio Culture, Stool.

2. If susceptibility testing is needed; also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, MIC. Pathogens for which susceptibility testing would be pursued are: Shigella species, Yersinia species, Campylobacter species, Arcobacter species, and Vibrio species. If susceptibilities are not appropriate they will not be performed and ZMMLS will be canceled at report time.

3. Clostridium difficile, a major cause of nosocomial diarrhea, is not detected by this test (order CDFRP / Clostridium difficile Toxin, Molecular Detection, PCR, Feces).

4. Escherichia coli O157:H7 is not detected by this test (Order STFRP / Shiga Toxin, Molecular Detection, PCR, Feces).

Shipping Instructions

Specimen must arrive within 96 hours of collection.

Necessary Information

Specimen source is required.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Patient Preparation: Medications: Do not use antacids, barium, bismuth, antidiarrheal medication, or oily laxatives before collection of specimen.

Supplies: C and S vial (T058)

Specimen Type: Preserved Stool

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S [T058])

Specimen Volume: Representative portion of stool specimen

Collection Instructions:

1. Collect fresh stool and submit 1 gram or 5 mL in container with transport medium.

2. Place stool in preservative within 2 hours of collection.

3. Place vial in a sealed plastic bag.

Forms

If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Test Request Form (T728) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/gastroenterology-and-hepatology-test-request.pdf)

Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Specimen >96 hours old, unpreserved stool, ECOFIX preservative, formalin, or PVA fixative

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
FecalAmbient (preferred)4 days
 Refrigerated 4 days