TWRP - Specimen: Tropheryma whipplei, Molecular Detection, PCR

Test Catalog

Test Name

Test ID: TWRP    
Tropheryma whipplei, Molecular Detection, PCR

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

See Infectious Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology in Special Instructions.

Specimen Type Describes the specimen type validated for testing

Varies

Necessary Information

Specimen source is required.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Tropheryma whipplei DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Specimen Type: Fresh tissue or biopsy

Sources: Small intestine tissue (duodenum, ileum, or jejunum), lymph node, other visceral tissue, synovial tissue, gastrointestinal tissue, heart valve, or brain

Container/Tube: Sterile container

Specimen Volume: Entire collection or 5 mm(3)

Collection Instructions: Collect fresh tissue specimen

Specimen Stability Information: Refrigerated <7 days (preferred)/Frozen <7 days

 

Supplies: Tissue Block Container (T553)

Specimen Type: Paraffin-embedded tissue block

Sources: Small intestine tissue (duodenum, ileum, or jejunum), lymph node, other visceral tissue, synovial tissue, gastrointestinal tissue, heart valve, or brain

Container/Tube: Sterile container for cut sections or Tissue Block Container (T553)

Collection Instructions: Submit formalin-fixed, paraffin-embedded tissue block to be cut and returned.  Alternately, perform cuts and place two to five 10-micron sections in a sterile container for submission

Specimen Stability Information: Ambient (preferred)/Refrigerated

 

Specimen Type: Fluid

Sources: Cerebrospinal or ocular (eg, vitreous humor)

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Specimen Stability Information: Refrigerated <7 days (preferred)/Frozen <7 days

 

Specimen Type: Synovial fluid

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Pink top (EDTA), royal blue top (EDTA), sterile vial containing EDTA-derived aliquot, red clot tube (no anticoagulant), or sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Send specimen in original tube (preferred).

Specimen Stability Information: Refrigerated <7 days (preferred)/Frozen <7 days

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Microbiology Test Request Form (T244) (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)

Gastroenterology and Hepatology Test Request Form (T728) (http://www.mayomedicallaboratories.com/it-mmfiles/gastroenterology-and-hepatology-test-request.pdf)

Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

Spinal fluid, synovial fluid, or vitreous humor fluid: 0.5 mL
Fresh tissue or biopsy: 5 mm(3)
Paraffin-embedded tissue block: two 10-micron sections

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Anticoagulants other than EDTA, green top (heparin) tube, blue top (citrate) tube, yellow top (ACD) tube, serum separator tube (SST); slides, bone marrow

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperatures are also included.

Specimen TypeTemperatureTime
VariesVaries