GRAM - Specimen: Gram Stain

Test Catalog

Test Name

Test ID: GRAM    
Gram Stain

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

See Infectious Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology in Special Instructions.

Specimen Type Describes the specimen type validated for testing


Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Specimen sources: Closed/open abscess, lower respiratory, fluid, tissue, or swab


Supplies: Culturette (BBL Culture Swab) (T092)

Container/Tube: Sterile container or culture transport swab (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium [T092])

Specimen Volume: Entire collection



Slides: Prepared microscope slide

Collection Container/Tube: Sterile container or culture transport swab

Submission Container/Tube: Slide container

Collection Instructions: Apply original sample to surface of standard microscope slide using appropriate application method (determined by consistency of specimen type) to assure adequate transfer of specimen onto slide. Allow specimen to dry and then heat-fix the slide. Place in slide container for transport.

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected








Blood, stool, vaginal/cervical secretions, throat, or nasal specimen.

Specimen >24 hours or frozen


Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperatures are also included.

Specimen TypeTemperatureTime
VariesRefrigerated (preferred)24 hours
 Ambient 24 hours