SPN - Specimen: Pneumocystis Smear

Test Catalog

Test Name

Test ID: SPN    
Pneumocystis Smear

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, the reflex test may be performed and charged.

Specimen Type Describes the specimen type validated for testing


Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Specimen source is required.


Submit only 1 of the following specimens:



Specimen Type: Bronchoalveolar lavage

Container/Tube: Sterile container

Specimen Volume: Minimum of 2 mL


Specimen Type: Lung or open lung tissue

Container/Tube: Sterile container

Specimen Volume: Minimum of a rice size piece

Collection Instructions: Tissue should be placed in small amount of sterile saline or sterile water.



Specimen Type: Bronchial washing, sputum, or tracheal secretion

Container/Tube: Sterile container

Specimen Volume: Minimum of 1 mL


Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

Brochoalveolar lavage: 2mL/Sputum, bronchial washings, and tracheal secretions: 1 mL/Lung tissue and open lung biopsy: rice-sized piece of tissue

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected








Fixed tissue; viral transport media (including but not limited to M4, M5, BD viral transport media, thioglycolate broth); wood shaft or charcoal swab


Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperatures are also included.

Specimen TypeTemperatureTime
VariesRefrigerated (preferred)7 days
 Ambient 7 days