GAL1P - Specimen: Galactose-1-Phosphate (Gal-1-P), Erythrocytes

Test Catalog

Test Name

Test ID: GAL1P    
Galactose-1-Phosphate (Gal-1-P), Erythrocytes

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

See Galactosemia Testing Algorithm in Special Instructions

Specimen Type Describes the specimen type validated for testing

Washed RBC

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

Erythrocytes must be washed within 4 hours of draw.


Collection Container/Tube: Green top (heparin)

Submission Container/Tube: Plastic vial

Specimen Volume: Washed, packed cells (red cell pellet from step f in Collection Instructions)

Collection Instructions:

1. Collect and process a minimum of 2 mL of whole blood as follows:

a. Immediately centrifuge for 10 minutes at 650 x G. For conversion to RPMs, see Additional Information.

b. Discard the plasma and buffy coat layers.

c. Add a cold 0.9% saline solution to the erythrocytes (about 2 times the volume of erythrocytes).

d. Mix gently by inversion and centrifuge again for 10 minutes at 650 x G.

e. Remove and discard the saline.

f. Repeat the wash steps (steps c-e) 2 more times.

2. After the final centrifugation, remove and discard the saline and a thin layer of the top cells.

Additional Information:

1. For infants, collect specimen immediately prior to feeding to avoid postprandial elevations.

2. The relative centrifugal force (G-force) can be estimated by applying the following formula: g=11.18 x r x (n/1000)(2).

Where: r=radius in centimeters and n=speed in RPM.

The radius from the center of the rotation axis to the bottom or outermost portion of the test tube should be used. RCF is expressed relative to the force of the earth's gravity.

Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

2 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected









Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperatures are also included.

Specimen TypeTemperatureTime
Washed RBCFrozen9 days