LPA3P - Specimen: Lymphocyte Proliferation to Anti-CD3/Anti-CD28 and Anti-CD3/Interleukin-2 (IL-2) by Flow Cytometry

Test Catalog

Test Name

Test ID: LPA3P    
Lymphocyte Proliferation to Anti-CD3/Anti-CD28 and Anti-CD3/Interleukin-2 (IL-2) by Flow Cytometry

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

Testing with 2 stimulants will always be performed. When adequate specimen is available, a third stimulant will be performed and billed separately. If isolated patient’s peripheral blood mononuclear cells (PBMCs) have a low WBC, selected dilutions or stimulants may not be used at the discretion of the laboratory to ensure the most reliable results.

Specimen Type Describes the specimen type needed for testing

WB Sodium Heparin

Shipping Instructions

Specimens are required to be received in the laboratory weekdays and by 4 p.m. on Friday. It is recommended that specimens arrive within 24 hours of draw. Specimens arriving on the weekend may be canceled. Draw and package specimen as close to shipping time as possible. Ship specimen overnight in an Ambient Mailer-Critical Specimens Only (T668).

Necessary Information

Date and time of draw and ordering physician's name and phone number are required.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

For serial monitoring, we recommend that specimen draws be performed at the same time of day.

 

Supplies: Ambient Mailer-Critical Specimens Only (T668)

Container/Tube: Green top (sodium heparin)

Specimen Volume:

<3 months: 1 mL

3 months-5 years: 3 mL

6-18 years: 5 mL

>18 years: 20 mL

Collection Instructions: Send specimen is original tube. Do not aliquot.

 

Blood Volume Recommendations Based on Absolute Lymphocyte Count (ALC)

 

ALC

Blood Volume for Minimum aCD28 Only

Blood Volume for Minimum of aCD3, aCD28, and IL-2

Blood Volume for Full Assay

<0.5

>15 cc

>28 cc

>50 cc

0.5-1.0

15 cc

28 cc

50 cc

1.1-1.5

6.5 cc

12 cc

24 cc

1.6-2.0

4.5 cc

8.5 cc

16 cc

2.1-3.0

3.5 cc

6.5 cc

12 cc

3.1-4.0

2.5 cc

4.5 cc

8 cc

4.1-5.0

1.8 cc

3.5 cc

6 cc

>5.0

1.5 cc

2.5 cc

5 cc

Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross OK

Icterus

NA

Other

Lithium heparin tube

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperature(s) are also included.

Specimen TypeTemperatureTime
WB Sodium HeparinAmbient48 hours