CDFRP - Specimen: Clostridium difficile Toxin, Molecular Detection, PCR, Feces

Test Catalog

Test Name

Test ID: CDFRP    
Clostridium difficile Toxin, Molecular Detection, PCR, Feces

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

See Laboratory Testing for Infectious Causes of Diarrhea in Special Instructions.

Specimen Type Describes the specimen type validated for testing


Necessary Information

Specimen source is required.

Specimen Required Defines the optimal specimen. This field describes the type of specimen required to perform the test and the preferred volume to complete testing. The volume allows automated processing, fastest throughput and, when indicated, repeat or reflex testing.

This test is validated for formed stool, although testing formed stool for Clostridium difficile is generally not clinically indicated.


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Clostridium difficile Toxin DNA is unlikely.


Submit only 1 of the following specimens:



Specimen Type: Preserved stool

Supplies: C and S Vial (T058)

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of nonnutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S [T058])

Specimen Volume: Representative portion of stool 

Collection Instructions: Collect fresh stool and submit 1 gram or 5 mL in container with transport medium. Place stool in preservative within 2 hours of collection.



Specimen Type: Unpreserved stool

Supplies: Stool container, Small (Random), 4 oz (T288); Stool Collection Kit, Random (T635)

Container/Tube: Stool container (T288)

Specimen Volume: Representative portion of stool


If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Microbiology Test Request Form (T244) (

Gastroenterology and Hepatology Test Request Form (T728) (

Specimen Minimum Volume The amount of sample necessary to provide a clinically relevant result as determined by the Laboratory.

1 mL

Reject Due To Identifies specimen types and conditions that may cause the specimen to be rejected








Stool in gel transport medium; ECOFIX preservative; formalin or PVA fixative

Specimen Stability Information Provides a description of the temperatures required to transport a specimen to the laboratory. Alternate acceptable temperatures are also included.

Specimen TypeTemperatureTime
FecalAmbient (preferred)7 days
 Frozen 7 days
 Refrigerated 7 days