NPM1 - Performance: Nucleophosmin (NPM1) Mutation Analysis

Test Catalog

Test Name

Test ID: NPM1    
Nucleophosmin (NPM1) Mutation Analysis

Method Description Describes how the test is performed and provides a method-specific reference

DNA is extracted from blood or bone marrow and a PCR assay is performed using primers that amplify a fragment of NPM1 DNA containing the region susceptible to insertion mutation. One of the PCR primers contains a fluorescent tag. The amplified fragments are separated by capillary electrophoresis on an ABI3130 genetic analyzer. In unmutated DNA, the PCR fragments obtained are 187-base pairs (bp). PCR fragments containing an insertional mutation are longer, usually 191-bp, as the majority of mutations are 4-bp insertions.(Unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 3 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

4 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

6 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

Until reported

Performing Laboratory Location The location of the laboratory that performs the test