HCMM - Performance: Homocysteine (Total), Methylmalonic Acid, and Methylcitric Acid, Blood Spots

Test Catalog

Test Name

Test ID: HCMM    
Homocysteine (Total), Methylmalonic Acid, and Methylcitric Acid, Blood Spots

Method Description Describes how the test is performed and provides a method-specific reference

A single 3/16-inch dried blood spot punch is extracted with an acidified acetonitrile and water solution containing dithiotheritol, d3-methylmalonic acid, d3-methycitric acid, and d8-homocystine. During 1 hour of agitation, free homocystine, protein-bound homocysteine, and the added d8-homocystine internal standard are reduced to homocysteine. The extract is transferred and then evaporated under heated nitrogen. Dried residue is treated with 3 N HCl in n-butanol to form butylesters. After evaporation of the butanol, the residue is reconstituted, centrifuged, and the supernatant is transferred to microvials and subjected to liquid chromatography-tandem mass spectrometry analysis.(Turgeon CT, Magera MJ, Cuthbert CD, et al: Determination of total homocysteine, methylmalonic acid, and 2-methylcitric acid in dried blood spots by tandem mass spectrometry. Clin Chem 2010 Nov;56[11]:1686-1695)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday, Thursday; 8 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

2 years

Performing Laboratory Location The location of the laboratory that performs the test