F2ISO - Performance: F2-Isoprostanes, Urine

Test Catalog

Test Name

Test ID: F2ISO    
F2-Isoprostanes, Urine

Method Description Describes how the test is performed and provides a method-specific reference

F2-isoprostane (15-F2t-IsoP) and prostaglandin F2 alpha (PGF2 alpha) are separated and quantified in urine by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Deuterium-labeled 15-F2t-IsoP and PGF2 alpha internal standards are added to the samples which are then positive-pressure filtered to remove particulates and sediment from the urine. A Cyclone MAX mixed mode anion exchange turboflow column is used for sample clean-up, while a Waters Xbridge C8 analytical column is used for separation of 15-F2t-IsoP and PGF2 alpha. From this column, the sample is transferred to an API 5000 MS/MS for instrumental analysis. The ratios of the extracted peak areas of 15-F2t-IsoP and PGF2 alpha to their respective deuterium labeled internal standards are used to calculate the concentration of the respective analyte present. PGF2 alpha is not clinically reported, as its analysis is conducted only to ensure complete separation from 15-F2t-IsoP.(Milne GL, Sanchez SC, Musiek, ES, Morrow JD: Quantification of F2-isoprostanes as a biomarker of oxidative stress. Nature Prot 2007;2:221-226)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Wednesday; 11 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

9 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location The location of the laboratory that performs the test