NEFA - Performance: Free Fatty Acids, Total, Serum

Test Catalog

Test Name

Test ID: NEFA    
Free Fatty Acids, Total, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Unesterified (free) fatty acids are measured by direct analysis of serum. Serum free fatty acids react with CoA in the presence of ATP, Mg(++), and acyl-CoA synthetase to form thiol esters of CoA. The esters are oxidized by acyl-CoA oxidase to produce hydrogen peroxide, which reacts with a signal compound to produce purple adduct. The free fatty acid concentration is proportional to the absorbance at 550 nm. Ascorbate oxidase is included as a reagent to completely remove any ascorbic acid in the specimen, which would interfere with the oxidative reactions.(Unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday; 7 a.m.-5 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day (not reported on Saturday or Sunday)

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location The location of the laboratory that performs the test