CTSA - Performance: Ceramide Trihexosides and Sulfatides, Urine

Test Catalog

Test Name

Test ID: CTSA    
Ceramide Trihexosides and Sulfatides, Urine

Method Description Describes how the test is performed and provides a method-specific reference

Ceramide trihexosides and sulfatides are determined by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Urine specimens are extracted with 2:1 chloroform: methanol. After evaporation, the dry residue is reconstituted in 10 mM ammonium formate in 90:10 methanol: CLR H20 + 0.05% formic acid for ceramide trihexosides analysis and methanol for sulfatide analysis. LC-MS/MS is performed using a mobile phase composed of 10 mM ammonium formate in 90:10 methanol: CLR H20 + 0.05% formic acid using a short C8 column (50 mm x 2.1 mm, 3.5 microns) to separate the ceramide trihexosides and sulfatides from the bulk of the specimen matrix. The MS/MS is operated in the multiple reaction monitoring (MRM) positive mode to follow the ceramide trihexosides and multiple reaction monitoring (MRM) negative mode to follow the sulfatides.(Unpublished Mayo method)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.


Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

7 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

14 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 month

Performing Laboratory Location The location of the laboratory that performs the test