CYCL - Performance: Cyclospora Stain

Test Catalog

Test Name

Test ID: CYCL    
Cyclospora Stain

Method Description Describes how the test is performed and provides a method-specific reference

A thin smear of the fecal concentrate is placed on a slide and allowed to air dry. The slide is then placed in acidic alcohol for 5 minutes, followed by washing with cold tap water. The slide is then placed in a Coplin jar containing safranin solution in acidified water and heated in a microwave at full power for 1 minute. Excess stain is washed off with tap water and the slide is then placed in a Coplin jar containing the counterstain (aqueous solution of either 1% methylene blue or 1% malachite green) for 1 minute. After a final rinse in tap water, the slide is dried and a coverslip is applied. The slide is examined microscopically using high-power with oil immersion. The oocysts of Cyclospora will be clear to pink and have a somewhat refractile quality.(Visvesvara GS, Moura H, Kovacs-Nace E, et al: Uniform staining of Cyclospora oocysts in fecal smears by a modified safranin technique with microwave heating. J Clin Microbiol 1997;35:730-733)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Saturday; 12 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

3 days

Performing Laboratory Location The location of the laboratory that performs the test