HIS - Performance: Histone Autoantibodies, Serum

Test Catalog

Test Name

Test ID: HIS    
Histone Autoantibodies, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Purified histones are bound to the wells of a polystyrene microwell plate under conditions that will preserve the antigen in its native state. Prediluted controls and diluted patient sera are added to separate wells. After washing, an anti-human IgG conjugated to horseradish peroxidase is added. After incubation and washing, tetramethylbenzidine (TMB) substrate is added to enable visualization of antibodies. The enzyme reaction is stopped and color development measured at 450 nm in a microtiter plate spectrophotometer.(Package insert: QUANTA Lite. Histone ELISA 708520, INOVA Diagnostics, Inc.)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday, Wednesday, Friday; 3:00 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

7 days

Performing Laboratory Location The location of the laboratory that performs the test