A1ALC - Performance: Alpha-1-Antitrypsin Proteotype S/Z by LC-MS/MS, Serum

Test Catalog

Test Name

Test ID: A1ALC    
Alpha-1-Antitrypsin Proteotype S/Z by LC-MS/MS, Serum

Method Description Describes how the test is performed and provides a method-specific reference

Proteins from patient sera are denatured, reduced, and digested with trypsin to form peptides. Labeled internal standards are added to the peptide mixture and subjected to selective reaction monitoring (SRM) liquid chromatograph-tandem mass spectrometry (LC-MS/MS) analysis. The presence or absence of the S and Z mutated peptides are determined by SRM peptide-specific m/z values for both the mutated and nonmutated peptides.(Chen Y, Snyder MR, Zhu Yi, et al: Simultaneous phenotyping and quantification of alpha-1-antitrypsin by liquid chromatography-tandem mass spectrometry. Clin Chem 2011;57[8]:1161-1168)


Nephelometry.(Siemens Nephelometer II Operations Instruction Manual. Siemens, Inc., Newark, DE)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information


Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday, Thursday; 12 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

7 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

14 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location The location of the laboratory that performs the test