HAEVP - Overview: Hemolytic Anemia Evaluation

Test Catalog

Test Name

Test ID: HAEVP    
Hemolytic Anemia Evaluation

Useful For Suggests clinical disorders or settings where the test may be helpful

Evaluation of lifelong or inherited hemolytic anemias, including red cell membrane disorders, unstable or abnormal hemoglobin variants, and red cell enzyme disorders


Cold agglutinin disorders and autoimmune disorders should be excluded prior to testing. This evaluation is not suitable for acquired causes of hemolysis.

Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test IDReporting NameAvailable SeparatelyAlways Performed
HAEVHemolytic Anemia InterpretationNoYes
A2FHemoglobin A2 and FNoYes
HBELHemoglobin Electrophoresis, BNoYes
UNHBUnstable Hemoglobin, BNoYes
FRAGOOsmotic FragilityNo, (Order FRAG)Yes
SCTRLShipping Control VialNoYes
PKPyruvate Kinase, RBCYesYes
GPIGlucose Phosphate Isomerase, BYesYes
HEXKHexokinase, BNoYes
PBSMMorphology ReviewNoYes

Reflex Tests Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests

Test IDReporting NameAvailable SeparatelyAlways Performed
GLTIGlutathione, BNoNo
HPFHHemoglobin F, Red Cell Distrib, BYesNo
SDEXHemoglobin S, Scrn, BYesNo
IEFIEF ConfirmsNoNo
MASSHb Variant by Mass Spec, BNoNo
RBCEReflexed RBC EnzymesNoNo
HGBMOHGB Electrophoresis, MolecularNoNo

Additional Tests Lists tests that are always performed, at an additional charge, with the initial tests

Test IDReporting NameAvailable SeparatelyAlways Performed
BND3Band 3 Fluorescence Staining, RBCNoYes

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

The most common RBC enzymes (G6PD / Glucose-6-Dehydrogenase (G-6-PD), Quantitative, Erythrocytes; PK / Pyruvate Kinase, Erythrocytes; GPI / Glucose Phosphate Isomerase, Erythrocytes; and HEXK / Hexokinase, Blood) will always be performed. If these are normal, the second tier enzymes will be performed (provided sufficient sample volume). If second tier enzymes are desired even if the first tier is abnormal, please fill out the Hemolytic Anemia Patient Information sheet (T705) and indicate this desire. HPLC, capillary electrophoresis, and hemoglobin stability studies will always be performed. Reflex testing required to identify a hemoglobin abnormality can be added as the case requires. Osmotic fragility and eosin-5-maleimide (EMA) binding (band 3) flow cytometry will be performed on all cases. A normal shipping control for osmotic fragility (OF) is necessary to exclude false positive results due to preanalytical artifact.


OF and EMA binding testing will be canceled if no shipping control is received or if the shipping control is abnormal.


This is a consultative evaluation in which the case will be evaluated and appropriate tests performed, at an additional charge, and the results interpreted. If a peripheral blood smear is provided, the morphologic features will be incorporated into the interpretation. If a Hemolytic Anemia Patient Information sheet (T705) is received with the sample, the reported clinical features or clinical impression will be incorporated into the interpretation.


For information on thalassemias and appropriate test ordering, see Thalassemia Tests in Special Instructions.


Note: RBCE / Reflexed RBC Enzymes, Blood (second-tier enzymes) includes: adenosine deaminase, adenylate kinase, phosphofructokinase, phosphoglycerate kinase, triosephosphate isomerase, and pyrimidine 5' nucleotidase.

Special Instructions and Forms Library of PDFs including pertinent information and consent forms, specimen collection and preparation information, test algorithms, and other information pertinent to test

Method Name A short description of the method used to perform the test

HAEV: Consultative Interpretation

A2F: Cation Exchange/High-Performance Liquid Chromatography (HPLC)

HBEL: Capillary Electrophoresis

UNHB: Isopropanol Stability

FRAGO: Osmotic Lysis

G6PD, PK, GPI, HEXK, RBCE, GLTI: Kinetic Spectrophotometry (KS)

PBSM: Consultant Review

BND3 (eosin-5-maleimide (EMA) binding): Flow Cytometry

MASS: Mass Spectrometry (MS)

IEF: Electrophoresis

HGBMO: Polymerase Chain Reaction (PCR) Analysis/Multiplex Ligation-Dependent Probe Amplification (MLPA), Polymerase Chain Reaction (PCR)/DNA Sequencing

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

Hemolytic Anemia Evaluation

Aliases Lists additional common names for a test, as an aid in searching

Hemolytic Anemia
EMA binding
Eosin 5 maleimide
RBC membrane
RBC enzyme
Unstable hemoglobin
Pyruvate kinase deficiency
Neonatal anemia
Neonatal hemolysis
Hereditary pyropoikilocytosis
Hereditary spherocytosis