HBELC - Overview: Hemoglobin Electrophoresis Cascade, Blood

Test Catalog

Test Name

Test ID: HBELC    
Hemoglobin Electrophoresis Cascade, Blood

Useful For Suggests clinical disorders or settings where the test may be helpful

Diagnosis of thalassemias and hemoglobin variants


Evaluation of unexplained microcytosis

Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test IDReporting NameAvailable SeparatelyAlways Performed
A2FHemoglobin A2 and FNoYes
HBELHemoglobin Electrophoresis, BNoYes

Reflex Tests Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests

Test IDReporting NameAvailable SeparatelyAlways Performed
HPFHHemoglobin F, Red Cell Distrib, BYesNo
SDEXHemoglobin S, Scrn, BYesNo
IEFIEF ConfirmsNoNo
MASSHb Variant by Mass Spec, BNoNo
HGBMOHGB Electrophoresis, MolecularNoNo
UNHBUnstable Hemoglobin, BNoNo

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

Hemoglobin electrophoresis cascade will always include hemoglobin A(2) and F and hemoglobin electrophoresis.


Reflex testing-Hemoglobin electrophoresis reflex testing, performed at additional charge, may include any or all of the following as indicated to identify rare hemoglobin variants present: hemoglobin S screen, unstable hemoglobin, isoelectric focusing confirms, hemoglobin variant by mass spectrometry, hemoglobin F red cell distribution, beta-globin gene, large deletion/duplication, alpha-globin gene sequencing, and beta-globin gene sequencing.

Special Instructions and Forms Library of PDFs including pertinent information and consent forms, specimen collection and preparation information, test algorithms, and other information pertinent to test

Method Name A short description of the method used to perform the test

A2F: Cation Exchange/High-Performance Liquid Chromatography (HPLC)

HBEL: Capillary Electrophoresis

IEF: Isoelectric Focusing

MASS: Mass Spectrometry (MS)

HGBMO: Polymerase Chain Reaction (PCR) Analysis/Multiplex Ligation-Dependent Probe Amplification (MLPA), Polymerase Chain Reaction (PCR)/DNA Sequencing

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

HGB Electrophoresis Cascade

Aliases Lists additional common names for a test, as an aid in searching

A2 Hemoglobin
Alpha Globin Variant
Barts Hemoglobin
Beta Globin Variant
Fetal Hemoglobin
fetaldex (Hemoglobin F)
Hemoglobin A2
Hemoglobin Cascade
Hemoglobin Electrophoresis Cascade Level 1
Hemoglobin F
Hemoglobin Mass Spec Studies
Hemoglobin Molecular studies
Hemoglobin S, quant
Hemoglobin Variant
Hemoglobin-H Disease
HGB (Hemoglobin) Electrophoresis
Isoelectric Focusing
Sickling Test