MPNR - Overview: Myeloproliferative Neoplasm (MPN), JAK2 V617F with Reflex to CALR and MPL

Test Catalog

Test Name

Test ID: MPNR    
Myeloproliferative Neoplasm (MPN), JAK2 V617F with Reflex to CALR and MPL

Useful For Suggests clinical disorders or settings where the test may be helpful

Aiding in the distinction between a reactive cytosis and a chronic myeloproliferative disorder


Evaluates for mutations in JAK2, CALR, and MPL in an algorithmic process

Reflex Tests Lists tests that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial tests

Test IDReporting NameAvailable SeparatelyAlways Performed
CALXCALR, Gene Mutation, Exon 9, ReflexNo, (bill only)No
MPLRMPL Exon 10 Mutation Detection, RNo, (bill only)No

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

This reflex test sequentially evaluates for the common major gene mutations associated with non-BCR/ABL1-positive myeloproliferative neoplasms until a mutation is identified. The testing sequence is based on the reported frequency of gene mutations in this disease group. Initial testing evaluates for the presence of the JAK2 V617F mutation. If this result is negative or very low positive (0.06%-0.6%), testing proceeds with assessment for CALR mutations. If the CALR result is also negative, then testing proceeds to evaluate for mutations in exon 10 of the MPL gene. If either JAK2 V617F (<0.06%) or CALR mutations are detected in the process, the testing algorithm ends; therefore, the complete reflex is followed only in the event of sequential negative mutation. An integrated report is issued with the summary of test results.


The following algorithms are available in Special Instructions:

-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood

-Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation

Special Instructions and Forms Library of PDFs including pertinent information and consent forms, specimen collection and preparation information, test algorithms, and other information pertinent to test

Method Name A short description of the method used to perform the test

Point Mutation Detection in DNA Using Quantitative Polymerase Chain Reaction (PCR)

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name A shorter or abbreviated version of the Published Name for a test; an abbreviated test name

MPN (JAK2 V617F, CALR, MPL) Reflex

Aliases Lists additional common names for a test, as an aid in searching

Janus kinase 2 gene
Tyrosine Kinase Mutation
Essential Thrombocythemia
JAK2-negative Myeloproliferative Neoplasm
Myeloproliferative Disorder
Myeloproliferative Neoplasm (MPN)
Primary Myelofibrosis
MPL S505
Myeloproliferative leukemia virus oncogene