MPNR - Overview: Myeloproliferative Neoplasm (MPN), JAK2 V617F with Reflex to CALR and MPL

Test Catalog

Test Name

Test ID: MPNR    
Myeloproliferative Neoplasm (MPN), JAK2 V617F with Reflex to CALR and MPL

Useful For Suggests clinical disorders or settings where the test may be helpful

Aiding in the distinction between a reactive cytosis and a chronic myeloproliferative disorder

 

Evaluates for mutations in JAK2, CALR, and MPL in an algorithmic process

Reflex Tests Lists test(s) that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial test(s)

Test IDReporting NameAvailable SeparatelyAlways Performed
CALXCALR, Gene Mutation, Exon 9, ReflexNo, (bill only)No
MPLRMPL Exon 10 Mutation Detection, RNo, (bill only)No

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

This reflex test sequentially evaluates for the common major gene mutations associated with non-BCR/ABL1-positive myeloproliferative neoplasms until a mutation is identified. The testing sequence is based on the reported frequency of gene mutations in this disease group. Initial testing evaluates for the presence of the JAK2 V617F mutation. If this result is negative or very low positive (0.06%-0.6%), testing proceeds with assessment for CALR mutations. If the CALR result is also negative, then testing proceeds to evaluate for mutations in exon 10 of the MPL gene. If either JAK2 V617F (<0.06%) or CALR mutations are detected in the process, the testing algorithm ends; therefore, the complete reflex is followed only in the event of sequential negative mutation. An integrated report is issued with the summary of test results.

 

The following algorithms are available in Special Instructions:

-Myeloproliferative Neoplasm: A Diagnostic Approach to Peripheral Blood

-Myeloproliferative Neoplasm: A Diagnostic Approach to Bone Marrow Evaluation

Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test

Method Name A short description of the method used to perform the test

Point Mutation Detection in DNA Using Quantitative Polymerase Chain Reaction (PCR)

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

MPN (JAK2 V617F, CALR, MPL) Reflex

Aliases Lists additional common names for a test, as an aid in searching

83872-JAK2B
Janus kinase 2 gene
Tyrosine Kinase Mutation
CALR
Calreticulin
Essential Thrombocythemia
JAK2-negative Myeloproliferative Neoplasm
Myelofibrosis
Myeloproliferative Disorder
Myeloproliferative Neoplasm (MPN)
Primary Myelofibrosis
MPL S505
MPLW515
Myeloproliferative leukemia virus oncogene