ML1HM - Overview: MLH1 Hypermethylation Analysis, Tumor

Test Catalog

Test Name

Test ID: ML1HM    
MLH1 Hypermethylation Analysis, Tumor

Useful For Suggests clinical disorders or settings where the test may be helpful

An adjunct to MSIHC / Microsatellite Instability (MSI)/Immunohistochemistry (IHC) Profile-Lynch/Hereditary Nonpolyposis Colorectal Cancer (HNPCC) Screen, when colon or endometrial tumor demonstrates microsatellite instability (MSI-H) and loss of MLH1 protein expression, to help distinguish a somatic versus germline event prior to performing expensive germline testing


An adjunct to negative MLH1 germline testing in cases where colon or endometrial tumor demonstrates MSI-H and loss of MLH1 protein expression


Note: Mayo's preferred screening test (BRMLH / MLH1 Hypermethylation and BRAF Mutation Analyses, Tumor) includes both MLH1 promoter hypermethylation and BRAF V600E testing. Please note that test can only be performed on colon tumors.

Additional Tests Lists tests that are always performed, at an additional charge, with the initial tests

Test IDReporting NameAvailable SeparatelyAlways Performed
SLIRVSlide Review in MGNo, (Bill Only)Yes

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, slide review will always be performed at an additional charge.


See Hereditary Nonpolyposis Colorectal Cancer Testing Algorithm in Special Instructions.

Special Instructions and Forms Library of PDFs including pertinent information and consent forms, specimen collection and preparation information, test algorithms, and other information pertinent to test

Method Name A short description of the method used to perform the test

Polymerase Chain Reaction (PCR) Analysis

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.


Reporting Name A shorter or abbreviated version of the Published Name for a test; an abbreviated test name

MLH1 Hypermethylation Analys, Tumor

Aliases Lists additional common names for a test, as an aid in searching

MLH1 Hypermethylation
Promoter Hypermethylation