BRMLH - Overview: MLH1 Hypermethylation and BRAF Mutation Analysis, Tumor

Test Catalog

Test Name

Test ID: BRMLH    
MLH1 Hypermethylation and BRAF Mutation Analysis, Tumor

Useful For Suggests clinical disorders or settings where the test may be helpful

An adjunct to MSIHC / Microsatellite Instability (MSI)/Immunohistochemistry (IHC) Profile-Lynch/Hereditary Nonpolyposis Colorectal Cancer (HNPCC) Screen, when colon tumor demonstrates microsatellite instability (MSI-H) and loss of MLH1 protein expression, to help distinguish a somatic versus germline event prior to performing expensive germline testing

 

An adjunct to negative MLH1 germline testing in cases where colon tumor from the same patient demonstrates MSI-H and loss of MLH1 protein expression

Genetics Test Information Provides information that may help with selection of the correct test or proper submission of the test request

If this test is ordered in conjunction with the hereditary nonpolyposis colorectal cancer (HNPCC) screen (MSIHC / Microsatellite Instability [MSI]/Immunohistochemistry [IHC] Profile-Lynch/Hereditary Nonpolyposis Colorectal Cancer [HNPCC] Screen) or MLH1 immunostain (IHC / Mismatch Repair [MMR] Protein Immunohistochemistry Only, Tumor ) and MSI (MSI / Microsatellite Instability [MSI], Tumor ), this test will only be performed when clinically indicated.

Profile Information A profile is a group of laboratory tests that are ordered and performed together under a single Mayo Test ID. Profile information lists the test performed, inclusive of the test fee, when a profile is ordered and includes reporting names and individual availability.

Test IDReporting NameAvailable SeparatelyAlways Performed
PBMLHMLH1 Hypermethylation/BRAF MutationNoYes

Reflex Tests Lists test(s) that may or may not be performed, at an additional charge, depending on the result and interpretation of the initial test(s)

Test IDReporting NameAvailable SeparatelyAlways Performed
BBRAFBRAF AnalysisYes, (order BRAFT)No
BMLHHMLH1 Hypermethylation AnalysisYes, (order ML1HM)No

Additional Tests Lists test(s) that are always performed, at an additional charge, with the initial test(s)

Test IDReporting NameAvailable SeparatelyAlways Performed
SLIRVSlide Review in MGNo, (Bill Only)Yes

Testing Algorithm Delineates situation(s) when tests are added to the initial order. This includes reflex and additional tests.

When this test is ordered, BRAF analysis and MLH1 hypermethylation analysis will always be performed. The exception would be if the tissue origin is an endometrial tumor; in those cases MLH1 hypermethylation analysis will be performed. If an endometrial tumor does not show loss of MLH1 by immunohistochemistry, we could still run BRAF and not do MLH1 hypermethylation.  

 

When this test is ordered, slide review will always be performed at an additional charge.

 

See Hereditary Nonpolyposis Colorectal Cancer Testing Algorithm in Special Instructions.

Special Instructions and Forms Describes specimen collection and preparation information, test algorithms, and other information pertinent to test. Also includes pertinent information and consent forms to be used when requesting a particular test

Method Name A short description of the method used to perform the test

Polymerase Chain Reaction (PCR) Analysis

(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.)

NY State Approved Indicates the status of NY State approval and if the test is orderable for NY State clients.

Yes

Reporting Name A shorter/abbreviated version of the Published Name for a test; an abbreviated test name

MLH1 Hypermethylation/BRAF Mutation

Aliases Lists additional common names for a test, as an aid in searching

BRAF Mutation
BRAF V600E
Hypermethylation
MLH1 Hypermethylation
Promoter Hypermethylation
MLBRF