SDEX - Clinical: Hemoglobin S, Screen, Blood

Test Catalog

Test Name

Test ID: SDEX    
Hemoglobin S, Screen, Blood

Useful For Suggests clinical disorders or settings where the test may be helpful

Screening for presence or absence of hemoglobin S (sickle cell disease)

Note: for quantification of hemoglobin S order HBELC / Hemoglobin Electrophoresis Cascade, Blood

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Homozygous hemoglobin S (sickle cell disease) is a serious chronic hemolytic anemia most commonly found in those of African or Middle Eastern descent.


Hemoglobin S is freely soluble when fully oxygenated; when oxygen is removed, polymerization of the abnormal hemoglobin occurs, forming tactoids that are rigid and deformed cells. This leads to sickling of the cells, hemolysis, and many other complications.


Heterozygous hemoglobin S (sickle cell trait) is the most common hemoglobinopathy in the United States. This condition is present in about 8% of African Americans. Usually, hemoglobin S trait exhibits no clinical or hematological effects. A small fraction of people with sickle cell trait have recurrent hematuria.

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.


Interpretation Provides information to assist in interpretation of the test results

A positive result should be followed by a complete hemoglobin evaluation (HBELC / Hemoglobin Electrophoresis Cascade, Blood) to confirm the presence and concentration of hemoglobin S.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

A positive test is presumptive evidence for hemoglobin (Hb) S (sickle cell disease). However, rare sickling hemoglobins such as Hb C-Harlem (C-Georgetown) and Hb I will also produce a positive result.


This test only detects the presence of Hb S. It cannot differentiate sickle cell trait (heterozygous Hb S) from sickle cell disease (homozygous Hb S), or Hb S in combination with other abnormalities (eg, S/C, S/D, S/G, S/E, S/beta-thalassemia, S/O-Arab, S/New York, and C-Georgetown trait).


The use of packed RBCs instead of whole blood significantly reduces false negatives due to anemia and false positives due to hypergammaglobulinemia (eg, multiple myeloma).


False positives can occur due to large numbers of nucleated RBCs.


False negatives can occur due to an insufficient quantity of Hb S due to age (neonates) or transfusion. Hb S concentrations 15% to 20% or less may give a negative result.

Clinical Reference Recommendations for in-depth reading of a clinical nature

Fairbanks VF: Laboratory methods and case studies. In Hemoglobinopathies and Thalassemias. Edited by BC Decker, New York, Thieme-Stratton Inc, 1980, pp 105-107