ABSCM - Clinical: Antibody Screen, Erythrocytes

Test Catalog

Test Name

Test ID: ABSCM    
Antibody Screen, Erythrocytes

Useful For Suggests clinical disorders or settings where the test may be helpful

Detection of allo- or autoantibodies directed against red blood cell antigens in the settings of pretransfusion testing


Evaluation of transfusion reactions


Evaluation of hemolytic anemia

Testing Algorithm Delineates situations when tests are added to the initial order. This includes reflex and additional tests.

If the antibody screen is positive, then antibody identification will be performed.

The following tests may also be ordered and performed as part of antibody identification: monospecific direct Coombs, special red cell antigen typing, antibody elution, and antibody absorption.

Clinical Information Discusses physiology, pathophysiology, and general clinical aspects, as they relate to a laboratory test

Transfusion and pregnancy are the primary means of sensitization to red cell antigens.


In a given population, 2% to 4% of the general population possess irregular red cell alloantibodies. Such antibodies may cause hemolytic disease of the newborn or hemolysis of transfused donor red blood cells.


Autoantibodies react against the patient's own red cells as well as the majority of cells tested. Autoantibodies can be clinically benign or can hemolyze the patient's own red blood cells, such as in cold agglutinin disease or autoimmune hemoyltic anemia.

Reference Values Describes reference intervals and additional information for interpretation of test results. May include intervals based on age and sex when appropriate. Intervals are Mayo-derived, unless otherwise designated. If an interpretive report is provided, the reference value field will state this.


If positive, antibody identification will be performed.

Interpretation Provides information to assist in interpretation of the test results

A positive result (antibody detected) necessitates antibody identification to establish the specificity and clinical significance of the antibody detected.


Negative–no antibody detected.

Cautions Discusses conditions that may cause diagnostic confusion, including improper specimen collection and handling, inappropriate test selection, and interfering substances

Clinical evaluation of antibodies identified is necessary to determine their potential for harm to the patient at this time and to assess appropriate action to be taken in the future.

Clinical Reference Recommendations for in-depth reading of a clinical nature

Technical Manual. 17th edition. 2011