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Test ID: GLP    
Glucagon, Plasma

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Method Description Describes how the test is performed and provides a method-specific reference

Big plasma glucagon (BPG), which is considered to be biologically inactive, is extracted using ethanol prior to assay of the specimen. This enables accurate measurement of the biologically active glucagon in the specimen.(Hendriks T: Radioimmunoassay of clinically meaningful components of circulating glucagon: removal of big plasma glucagon prior to assay. Clin Chim Acta 1984;140:301-307) Following ethanol extraction, glucagon reacts with an antiglucagon antibody that is attached to tiny polystyrene beads. After incubation and washing, a second detection antibody is added and attaches to any bead-bound glucagon, forming a sandwich assay. A streptavidin-phycoerythrin (SPE) tag binds to the glucagon-antibody complex. Laser-based fluorescent analysis of the resulting glucagon-antibody-SPE complex is performed on the Luminex 200 instrument.(Package insert: Human Endocrine LINCOplex Kit, HENDO-65K-Rev. 03/03/06)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday, Thursday; 10 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

2 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

6 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

90 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester