Sex Hormone-Binding Globulin (SHBG), Serum
Method Description Describes how the test is performed and provides a method-specific reference
IMMULITE 2000 sex hormone-binding globulin (SHBG) is a solid-phase, 2-site chemiluminescent immunoassay. A polystyrene bead is coated with a monoclonal antibody specific for sex hormone-binding globulin (SHBG). The patient sample, diluted 1 in 20, and alkaline phosphatase-conjugated anti-SHBG (polyclonal) are added to the bead and incubated. SHBG in the sample is bound to form an antibody sandwich complex. Unbound conjugate is then removed by washing, after which substrate is added. The chemiluminescent substrate, a phosphate ester of adamantly dioxetane, undergoes hydrolysis in the presence of alkaline phosphatase to yield an unstable intermediate. The continuous production of this substance results in the sustained emission of light. The photon output is proportional to the concentration of SHBG in the sample.(Package insert: IMMULITE SHBG 2000, July 2008)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 5 a.m.-12 a.m., Saturday; 6 a.m.-6 p.m.