CD4 T-Cell Recent Thymic Emigrants (RTE)
Method Description Describes how the test is performed and provides a method-specific reference
CD4 recent thymic emigrants are assessed in peripheral blood drawn in EDTA tubes and the test is performed as a single-tube assay. A panel of antibodies is used for the assay: CD3, CD4, CD31, CD45RA, and CD45RO, conjugated to various fluorochromes. The blood is incubated with the antibodies in the dark, followed by RBC lysis. Absolute counts are obtained using BD TruCount tubes. The sample is then centrifuged and resuspended in a paraformaldehyde solution for analysis on a BD FACS Canto A or Canto II flow cytometer. The data analysis is performed using BD FACS Diva software.(Unpublished Mayo method)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday
Do not send specimen after Thursday. Specimen must be received by 10 a.m. on Friday.