Fetal Lung Profile, Amniotic Fluid
Method Description Describes how the test is performed and provides a method-specific reference
This test uses a 1-dimensional, thin-layer chromatography (TLC) technique for separating the phospholipids in amniotic fluid. The first step in the process involves extraction of the lipids present in the amniotic fluid using a chloroform/methanol mixture. The extracted specimen is streaked onto a TLC plate and placed in tank 1 for prechromatography, a step that removes a component that otherwise comigrates with PG. Lipids are then separated using tank 2. The separated lipids are made visible with dilute cupric acetate and charring. L to S is determined using a scanning densitometer. The relative intensities of L and S are expressed as a ratio, and PG is reported from a visual observation of the plate and reported as present, absent, or trace. (Unpublished Mayo method)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 2 p.m.
Saturday and Sunday; 11 a.m.
Weekdays: Sample delivery time is 7:00 a.m. to 4:30 p.m.
Set-up time up to 2:00 p.m.
Saturday: Sample delivery time is 7:00 a.m. to 3:00 p.m.
Set-up time is up to 11:00 a.m.
Sunday: Sample delivery time is 8:00 a.m.-1:00 p.m.
Set-up time is up to 11:00 a.m. (On call only)
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test