Orotic Acid, Urine
Method Description Describes how the test is performed and provides a method-specific reference
Interfering substances such as amino acids, urea, pigments, and creatinine are removed from the urine by passing it through a cation-exchange resin. The orotic acid is then brominated to form dibromobarbituric acid, reduced to barbituric acid with ascorbic acid, and finally condensed with dimethylaminobenzaldehyde to form 5(p-dimethylaminobenzaldehyde)-barbituric acid. The absorbance of the final product is measured at 480 nm using a control reaction as reference.(Harris ML, Oberholzer VG: Conditions affecting the colorimetry of orotic acid and orotidine in urine. Clin Chem 1980;26:473-479)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Tuesday, Thursday; 8am
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test