1,25-Dihydroxyvitamin D, Serum
Method Description Describes how the test is performed and provides a method-specific reference
Deuterated stable isotopes (d3-1,25 dihydroxyvitamin D3 [DHVD3-d3] and d6-1,25 dihydroxyvitamin D2 [DHVD2-d6]) are added to a 1-mL plasma sample as internal standard. 1,25-Dihydroxyvitamin D2 (DHVD2), 1,25-dihydroxyvitamin D3 (DHVD3), and the internal standard are extracted using acetonitrile precipitation. The extracts are then further purified by SPE and affinity extraction. Extracts are then derivatized using 4-phenyl-1, 2,4-triazoline-3,5-dione and analyzed by chromatography-tandem mass spectrometry using multiple reaction monitoring with a C18 turbo cleanup for excess derivatizing reagent. DHVD2 and DHVD3 are quantified and a total is reported with a clinical reference range attached to the total DHVD.(Unpublished Mayo method)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 3 p.m.