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Test ID: MPSEX    
Monoclonal Protein Study, Expanded Panel, Serum

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Method Description Describes how the test is performed and provides a method-specific reference

Electrophoresis:

Serum proteins are separated in an electric field according to their size, shape, and electric charge. The separation is performed on agarose gels. The proteins are visualized by staining with acid blue and the intensity of staining is quantitated by densitometry (Helena Quick Scan 2000). Multiplying by the serum total protein converts the percentage of protein in each fraction into serum concentration.(Package insert: Helena SPIFE 3000 Instruction Manual and Helena SPIFE SPE Vis Gel 2001.)

 

Immunofixation: 

Immunofixation is performed with Sebia reagent sets and are specific for gamma, alpha, mu, kappa, and lambda immunoglobulin heavy and light chains. If a monoclonal light chain is detected in the absence of an associated monoclonal heavy chain, an immunofixation electrophoresis (IFE) specific for delta and epsilon chains is performed.(Katzmann JA, Kyle RA: Chapter 10: Immunochemical characterization of immunoglobulins in serum, urine, and cerebrospinal fluid. In Manual of Molecular and Clinical Laboratory Immunology. Seventh edition. Edited by B Detrick, RG Hamilton, JD Folds. Washington DC. ASM Press, 2006 pp 88-100)

                                                                                                                                                                            

Free Light Chains:

The quantitation of free light chain (FLC) by nephelometry uses FLC antisera from The Binding Site, Ltd., and is performed on the Siemens Nephelometer II.(Bradwell AR, Carr-Smith HD, Mead GP, et al: Highly sensitive, automated immunoassay for immunoglobulin free light chains in serum and urine. Clin Chem 2001;47[4]:673-80)

PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information

No

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Performing Laboratory Location The location of the laboratory that performs the test

Rochester