Congenital Adrenal Hyperplasia (CAH) Profile for 21-Hydroxylase Deficiency
Method Description Describes how the test is performed and provides a method-specific reference
Deuterated stable isotopes (d4-cortisol, d7-androstenedione, d8-17-hydroxyprogesterone) are added to a 0.1-mL serum sample as internal standards. Cortisol, androstenedione, 17-hydroxyprogesterone, and the internal standards are extracted from specimens using a Strata X 30-mg cartridge and eluted from the cartridge with methanol. The extracts are then dried down under nitrogen, reconstituted with 75 mcL of 70/30 methanol/H2O containing 1 g/mL of estriol and analyzed by liquid chromatography-tandem mass spectrometry using multiple-reaction monitoring. A calibration curve is generated by spiking standards into a bovine serum albumin buffer and extracted with each batch of new working internal standard. Controls are extracted with each batch.(Unpublished Mayo method)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 2 p.m.