T4 (Thyroxine), Total Only, Serum
Method Description Describes how the test is performed and provides a method-specific reference
The instrument used is a Beckman Coulter DXI 800. The Access total thyroxine (T4) assay is competitive-binding immunoenzymatic assay. A sample is added to a reaction vessel with antithyroxine antibody, thyroxine-alkaline phosphatase conjugate, and para-magnetic particles coated with goat antimouse capture antibody and a stripping agent to dissociate all T4 from binding proteins. Thyroxine in the sample competes with the thyroxine-alkaline phosphatase conjugate for binding sites on a limited amount of specific antithyroxine antibody. Resulting antigen: antibody complexes bind to the capture antibody on the solid phase. After incubation in a reaction vessel, materials bound to the solid phase are held in a magnetic field, while unbound materials are washed away. The chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is inversely proportional to the concentration of thyroxine in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve.(Instruction manual: Beckman Coulter Inc, Fullerton, CA 2009)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday 5 a.m. – 12 a.m., Saturday 6 a.m. – 6 p.m.