Test ID: FSH
Follicle-Stimulating Hormone (FSH), Serum
‹ Back to Pediatric index
The instrument used is Beckman Coulter Unicel DXI 800. The Access human follicle-stimulating hormone (hFSH) assay is a sequential 2-step immunoenzymatic sandwich assay. A sample is added to a reaction vessel with paramagnetic particles coated with goat antimouse:mouse anti-hFSH complexes and TRIS buffered saline with protein. The hFSH binds to the immobilized mouse anti-hFSH on the solid phase. Materials bound to the solid phase are held in a magnetic field, while unbound materials are washed away. Alkaline phosphatase conjugated goat anti-hFSH is then added and binds to the previously bound hFSH on the particles. A second separation and wash step removes unbound conjugate. The chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of hFSH in the sample. The amount of analyte in the sample is determined from a stored, multipoint calibration curve.(Instruction manual: Beckman Coulter, Inc., Fullerton, CA, 2010)
Monday through Friday; 5 a.m.-12 a.m., Saturday; 6 a.m.-6 p.m.
Same day/1 day