Test ID: LH
Luteinizing Hormone (LH), Serum
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The instrument used is the Beckman Coulter Unicel DXI 800. The Access hLH assay is a sequential 2-step immunoenzymatic sandwich assay. Sample is added to a reaction vessel, along with paramagnetic particles coated with goat antimouse:mouse anti-hLH complexes and TRIS buffered saline with protein. The hLH binds to the immobilized mouse anti-hLH on the solid phase. Materials bound to the solid phase are held in a magnetic field, while unbound materials are washed away. Alkaline phosphatase conjugated goat anti-hLH is then added, which binds to the previously bound hLH on the particles. A second separation and wash step removes unbound conjugate. The chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of hLH in the sample. The amount of analyte in the sample is determined from a stored multipoint calibration curve.(Beckman Assay Manual: Beckman Coulter Inc, Fullerton, CA, 2010)
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