Luteinizing Hormone (LH), Serum
Method Description Describes how the test is performed and provides a method-specific reference
The instrument used is the Beckman Coulter Unicel DXI 800. The Access hLH assay is a sequential 2-step immunoenzymatic sandwich assay. Sample is added to a reaction vessel, along with paramagnetic particles coated with goat antimouse:mouse anti-hLH complexes and TRIS buffered saline with protein. The hLH binds to the immobilized mouse anti-hLH on the solid phase. Materials bound to the solid phase are held in a magnetic field, while unbound materials are washed away. Alkaline phosphatase conjugated goat anti-hLH is then added, which binds to the previously bound hLH on the particles. A second separation and wash step removes unbound conjugate. The chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of hLH in the sample. The amount of analyte in the sample is determined from a stored multipoint calibration curve.(Beckman Assay Manual: Beckman Coulter Inc, Fullerton, CA, 2010)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 5 a.m.–12 a.m., Saturday; 6 a.m.–6 p.m.