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Test ID: LH    
Luteinizing Hormone (LH), Serum

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Method Description Describes how the test is performed and provides a method-specific reference

The instrument used is the Beckman Coulter Unicel DXI 800. The Access hLH assay is a sequential 2-step immunoenzymatic sandwich assay. Sample is added to a reaction vessel, along with paramagnetic particles coated with goat antimouse:mouse anti-hLH complexes and TRIS buffered saline with protein. The hLH binds to the immobilized mouse anti-hLH on the solid phase. Materials bound to the solid phase are held in a magnetic field, while unbound materials are washed away. Alkaline phosphatase conjugated goat anti-hLH is then added, which binds to the previously bound hLH on the particles. A second separation and wash step removes unbound conjugate. The chemiluminescent substrate Lumi-Phos 530 is added to the vessel and light generated by the reaction is measured with a luminometer. The light production is directly proportional to the concentration of hLH in the sample. The amount of analyte in the sample is determined from a stored multipoint calibration curve.(Beckman Assay Manual: Beckman Coulter Inc, Fullerton, CA, 2010)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday 5 a.m. – 12 a.m., Saturday 6 a.m. – 6 p.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

Same day/1 day

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

3 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

14 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester