Test ID: HYOX
Hyperoxaluria Panel, Urine
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Urine samples corresponding to 0.25 mg of creatinine (not to exceed 1 mL of urine) are oximated by reaction with methoxyamine hydrochloride to stabilize one of the target analytes, 4-hydroxy-2-oxoglutaric (HOG). The urine is then acidified and extracted with 4:1 ethyl acetate:isopropanol. After evaporation, the dry residue is silylated with 80:20 BSTFA/1%TMCS:pyridine and analyzed by capillary gas chromatography/mass spectrometry (GC/MS). In the context of hyperoxaluria, per se a non-specific finding, the detection of elevated excretions of urinary glycolate and glycerate allows the differential diagnosis between hyperoxaluria type 1 and type 2. Increased concentrations of oxalate and glycolate indicate type1 hyperoxaluria. Increased concentrations of oxalate and glycerate indicate type 2 hyperoxaluria. Increased concentration of HOG indicates type 3 hyperoxaluria, or carrier status for this disease. Increased concentrations of oxalate with normal concentrations of glycolate, glycerate, and HOG indicate secondary hyperoxaluria.(Unpublished Mayo method)
Varies; Batched 2 times per month; 8 a.m.