Hyperoxaluria Panel, Urine
Method Description Describes how the test is performed and provides a method-specific reference
Urine corresponding to 0.25 mg creatinine is aliquoted. Oxidation of 2-keto acids is performed by reaction with hydroxylamine hydrate. The urine is acidified and extracted with ethyl acetate. After evaporation, the dry residue is sialylated with N,O-Bis(trimethylsilyl)trifluoroacetamide (BSTFA)/1% trimethylchlorosilane (TMCS) and analyzed by capillary gas chromatography/mass spectrometry (GC/MS) for glycolate, glycerate, glyoxylate, and oxalate in single ion monitoring mode. Quantitation is performed against a known concentration of a stable isotope of each compound.(Mamer OA, Osei-Twum EY, Reimer MLJ, et al: Determination of oxalic, glycolic, glyoxylic, glyceric and hydroxypyruvic acids in a single GC/MS analysis useful for distinguishing hyperoxaluria types I and II. In Advances in Chemical Diagnosis and Treatment of Metabolic Disorders. Vol.2. Edited by I Matsumoto, T Kuhara, OA Mamer, et al. Kanazawa Medical Publishers, Limited 1994, pp 87-95)
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Varies; Batched 1 time per month; 8 a.m.
Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.
Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded
Performing Laboratory Location The location of the laboratory that performs the test