Test ID: T3
T3 (Triiodothyronine), Total, Serum
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Testing is performed on a Roche cobas instrument. The Roche triiodothyronine assay (T3) is a competitive assay using electrochemiluminescence detection. Bound T3 is released from binding proteins by 8-anilino-1-naphthalene sulfonic acid (ANS). The patient specimen is incubated with a sheep polyclonal anti-T3 antibody labeled with ruthenium. Streptavidin-coated microparticles and biotinylated T3 are added for a second incubation during which the still free binding sites of the labeled antibody become occupied. The resulting immunocomplex becomes bound to the solid phase by interaction of biotin and streptavidin. The reaction mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are removed and application of a voltage to the electrode induces the electrochemiluminescent emission. This signal is measured against a calibration curve to determine patient results.(Package insert: Roche cobas. Roche Diagnostics, Indianapolis, IN.)
Monday through Friday; 5 a.m.-12 a.m.
Saturday; 6 a.m.-6 p.m.
Same day/1 day