Dehydroepiandrosterone Sulfate (DHEA-S), Serum
Method Description Describes how the test is performed and provides a method-specific reference
The IMMULITE 2000 DHEA-SO4 is a solid-phase, chemiluminescent immunoassay. A polystyrene bead is coated with a polyclonal rabbit antibody specific for DHEA-SO4. The patient sample and alkaline phosphatase-conjugated DHEA-SO4 are added to the bead and incubated. During this time, DHEA-SO4 in the sample competes with the enzyme-labeled DHEA-SO4 for a limited number of antibody binding sites on the bead. Unbound enzyme conjugate is then removed by washing, after which substrate is added. The chemiluminescent substrate, a phosphate ester of adamantyl dioxetane, undergoes hydrolysis in the presence of alkaline phosphatase to yield an unstable intermediate. The continuous production of this intermediate substance results in the sustained emission of light. The photon output, is inversely proportional to the concentration of DHEA-SO4 in the sample.(Package insert: Immulite 2000 DHEA-SO4 [PIL2KDS-17], Siemens Medical Solutions, Los Angeles, CA 2008-07-29)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 5 a.m.-12 a.m., Saturday; 6 a.m.-6 p.m.