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Test ID: MPSSC    
Mucopolysaccharides (MPS) Screen, Urine

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Method Description Describes how the test is performed and provides a method-specific reference

Spectrophotometry (quantitative):

Centrifuged urine is mixed with a dimethylmethylene blue (DMB)/Tris hydroxymethlyaminomethane (TRIS) solution. The metachromatic dye DMB binds with glycosaminoglycans (GAG) and the complex formed changes the spectra of the dye. The pink color complex is measured at 520 nm.(deJong JG, Wevers RA, Liebrand-van Sambeek R: Measuring urinary glycosaminoglycans in the presence of protein; an improved screening procedure for mucopolysaccharidoses based on dimethylmethylene blue. Clin Chem 1992;38:803-807; Panin G, Naia S, Dall'Amico R, et al: Simple spectrophotometric quantification of urinary excretion of glycosaminoglycan sulfates. Clin Chem 1986;32:2073-2076)

 

Thin-Layer Chromatography (TLC):

Following precipitation, urinary GAG is separated by TLC based on the solubility of their calcium salts in varying ethanol concentration solvents. After separation, the sulfates are stained with Alcian blue for visualization.(Dembrune PP, Roesel RA: Screening for mucopolysaccharidoses by analysis of urinary glycosaminoglycans. In Techniques in Diagnostic Human Biochemical genetics-A Laboratory Manual. Edited by FA Hommes. New York, Wiley-Liss, 1991, pp 77-86)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday; 8am

 

MPS, quantitative: Monday through Friday; 12 p.m.

MPS, qualitative: Monday; 8 a.m.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

10 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

18 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

1 week

Performing Laboratory Location The location of the laboratory that performs the test

Rochester