Chromosome Analysis, Amniotic Fluid
Method Description Describes how the test is performed and provides a method-specific reference
The specimen is centrifuged and the cell pellet is mixed with culture media, then split into 6 primary culture dishes and a T-flask to establish cultures. Cells are harvested after 5 to 7 days. In the harvest process, the cells are exposed to ethidium bromide, colcemid, and hypotonic solution, and fixed with glacial acetic acid and methanol. Metaphase cells are dropped onto microscope slides and are routinely stained by G-banding, but other staining methods may be employed as needed. Twenty metaphases from 15 colonies and 3 or more primary cultures usually are examined. In cases where true mosaicism is suspected, up to 30 colonies and up to 6 primary cultures may be analyzed. Minimal evidence for the presence of an abnormality is defined as 2 or more metaphases with the same structural abnormality or chromosome gain (trisomy), or 3 or more metaphases lacking the same chromosome. Five or more digitized images of metaphases are stored in computer-based imaging systems and karyograms are prepared from 2 or more representative metaphases.(Chang HC, Jones OW: Amniocentesis: cell culture of human amniotic fluid in a hormone supplement. Cold Springs Harb Conf Cell Prolif 1982;9:1187-1192; Hsu LY, Perlis TE: United States survey on chromosome mosaicism and pseudomosaicism in prenatal diagnosis. Prenat Diagn 1984;4:97-130; Peakman DC, Moreton MF, Corn BJ, Robinson A: Chromosomal mosaicism in amniotic fluid cell cultures. Am J Hum Genet 1979;31:149-155; Spurbeck JL, Carlson RO, Allen JE, Dewald GW: Culturing and robotic harvesting of bone marrow, lymph nodes, peripheral blood, fibroblasts, and solid tumors with in situ techniques. Cancer Genet Cytogenet 1988;32:59-66)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Samples processed Monday through Sunday. Results reported Monday through Friday; 8 a.m.-5 p.m., CST.