Phenylalanine and Tyrosine, Plasma
Method Description Describes how the test is performed and provides a method-specific reference
This method quantifies phenylalanine (Phe) and tyrosine (Tyr) using stable isotope-labeled internal standards (IS): d5-Phe and d4-Tyr. Phe and Tyr are extracted from plasma using methanol:water (50:50) solution containing the IS. The mixture is vortexed and centrifuged to precipitate protein. The supernatant is diluted and then introduced into the tandem mass spectrometer (MS/MS). Chromatography is performed using a C18 (150x4.6mm) column. The concentration of Phe and Tyr are established by comparison of the ion intensity with that of the IS (d5-Phe and Tyr, respectively).(Unpublished Mayo method)
PDF Report Indicates whether the report includes an additional document with charts, images or other enriched information
Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday; 10 a.m.