Test ID: LPC
Lysophosphatidylcholine, Plasma
Method Description
Describes how the test is performed and provides a method-specific reference
Plasma is extracted with butanol after the addition of 17:0 lysophosphatidylcholine (LPC) as internal standard. After vortexing and centrifugation, the upper phase is collected, evaporated in a warm water bath using nitrogen, and reconstituted in methanol. Analysis is by electrospray liquid chromatography-tandem mass spectrometry (LC-MS/MS) operating in the positive mode and using selected-reaction monitoring. 16:0 and 18:0 LPCs are quantitated using 17:0 LPC as internal standard from calibration over a concentration range of 0.1 mcM to 5.0 mcM. (Lacey JM, Magera MJ, Matern D, et al: Rapid quantitative determination of lysophosphatidylcholine by liquid chromatography tandem mass spectrometry [LC-MS/MS]. J Am Soc Mass Spec 2003;13[5]S1:26S)
Day(s) and Time(s) Test Performed
Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Varies; 11 a.m.


