Test ID: PCT
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Procalcitonin (ProCT) is measured in this homogeneous automated immunofluorescent assay on the BRAHMS Kryptor. The Kryptor uses TRACE (Time Resolved Amplified Cryptate Emission) technology based on a nonradioactive transfer of energy. This transfer occurs between 2 fluorescent tracers: the donor (europium cryptate) and the acceptor (XL665). In the ProCT assay, a sheep polyclonal antibody against calcitonin is labeled with europium cryptate and a mouse monoclonal antibody against catacalcin is labeled with XL665. ProCT is sandwiched between the 2 antibodies, bringing them into close proximity. When the antigen-antibody complex is excited with a nitrogen laser at 337 nm, some fluorescent energy is emitted at 620 nm and the rest is transferred to XL665. This energy is then emitted as fluorescence at 665 nm. A ratio of the energy emitted at 665 nm to that emitted at 620 nm (internal reference) is calculated for each sample. Signal intensity is proportional to the number of antigen-antibody complexes formed, and therefore to antigen concentration.(Package insert: BRAHMS PCT Sensitive Kryptor. Thermo Fisher Scientific BRAHMS LLC, Middletown, VA)
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