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Test ID: ALPS    
Alpha Beta Double-Negative T Cells for Autoimmune Lymphoproliferative Syndrome

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Method Description Describes how the test is performed and provides a method-specific reference

This assay uses a 5-color, single-platform method with a 2-tube panel stained for the following antibodies: CD3, CD4, CD8, CD45, alpha beta TCR, and B220. The sample is stained with the antibody cocktail and incubated in the dark at room temperature for 20 minutes. Following incubation, the samples are treated with BD lysing solution to lyse the RBCs, followed with a wash step using BD FACS wash buffer. The cells are resuspended in 1% paraformaldehyde and analyzed by flow cytometry. The different subsets are expressed as a percent of CD3 T cells, and the absolute counts of all subsets are expressed as cells/mcL. (Unpublished Mayo method; Bleesing JJH, Brown MR, Dale JK, et al: TCR alpha beta + CD4-CD8- T-cells in humans with the autoimmune lymphoproliferative syndrome express a novel CD45 isoform that is analogous to murine B220 and represents a marker of altered O-glycan biosynthesis. Clin Immunol 2001;100[3]:314-324)

Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.

Monday through Friday

Do not send specimen after Thursday.

Specimen must be received by 10 a.m. Friday.

Analytic Time Defines the amount of time it takes the laboratory to setup and perform the test. This is defined in number of days. The shortest interval of time expressed is "same day/1 day," which means the results may be available the same day that the sample is received in the testing laboratory. One day means results are available 1 day after the sample is received in the laboratory.

3 days

Maximum Laboratory Time Defines the maximum time from specimen receipt at Mayo Medical Laboratories until the release of the test result

4 days

Specimen Retention Time Outlines the length of time after testing that a specimen is kept in the laboratory before it is discarded

3 days

Performing Laboratory Location The location of the laboratory that performs the test

Rochester