Activated Protein C Resistance V (APCRV), Plasma
Method Description Describes how the test is performed and provides a method-specific reference
This assay is performed using the Chromogenix COATEST activated protein C (APC) resistance V Kit on the ACL TOP instrument. The method uses a modified activated partial thromboplastin time (APTT) test to detect APC resistance. The plasma specimen is prediluted in factor V-deficient plasma. Then the APTT test is performed by incubating patient plasma with a standardized amount of platelet-like phospholipids and activator of the contact factors of the intrinsic coagulation pathway, followed by recalcification of plasma and measurement of clotting time.
The ratio of the APTT test with and without added APC is reported as the APC resistance (or sensitivity) ratio.(Kapiotis S, Quehenberger P, Jilma B, et al: Improved characteristics of APC resistance assay, COATEST APC resistance by predilution of samples with factor V deficient plasma. Am J Clin Pathol 1996;106:588-593; Fryburger G, Javorschi S, Labrouche S, Bernard P: Proposal for objective evaluation of the performance of various functional APC-resistance tests in genotyped patients. Thromb Haemost 1997;78:1360-1365; Hall C, Andersson N, Andras M, et al: Evaluation of a modified APTT-based method for determination of APC resistance in plasma from patients on heparin or oral anticoagulant therapy. Thromb Res 1998;89:203-209)
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Day(s) and Time(s) Test Performed Outlines the days and times the test is performed. This field reflects the day and time the sample must be in the testing laboratory to begin the testing process and includes any specimen preparation and processing time required before the test is performed. Some tests are listed as continuously performed, which means assays are performed several times during the day.
Monday through Friday